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dpnii restriction enzyme  (New England Biolabs)


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    Structured Review

    New England Biolabs dpnii restriction enzyme
    Dpnii Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1714 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dpnii+restriction+enzyme/Dpn+II/bio_rxiv__64898__2026__04__30__722071-397-17-20
    Average 99 stars, based on 1714 article reviews
    dpnii restriction enzyme - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Hi-C:

    Article Title: High-Resolution Genomic Resources for Trait Mapping and Precision Breeding for Adzuki Bean (Vigna angularis).
    Article Snippet: For sequencing the genome of “ZH20”, a 15 Kb library was constructed and sequenced using the PacBio Sequel II platform (Pacific Biosciences, CA, USA), generating a total of 41.36 Gb CCS HiFi reads with an N50 size of 22.31 Kb using CCS software (v3.0.0) (https://github.com/pacificbiosciences/unanimity/) using parameters “– min-passes 3 –min-length 10000 –max-length 1000000 –min-rq 0.99”. .. Additionally, Hi-C libraries were constructed using fresh leaf tissue fixed in 1% formaldehyde to extract chromatin, which was digested using the DpnII restriction enzyme (New England Biolabs, USA). .. After quality control, the Hi-C libraries were sequenced on an Illumina NovaSeq X platform (Illumina, CA, USA), yielding a total of 72.17 Gb Hi-C data.

    Construct:

    Article Title: High-Resolution Genomic Resources for Trait Mapping and Precision Breeding for Adzuki Bean (Vigna angularis).
    Article Snippet: For sequencing the genome of “ZH20”, a 15 Kb library was constructed and sequenced using the PacBio Sequel II platform (Pacific Biosciences, CA, USA), generating a total of 41.36 Gb CCS HiFi reads with an N50 size of 22.31 Kb using CCS software (v3.0.0) (https://github.com/pacificbiosciences/unanimity/) using parameters “– min-passes 3 –min-length 10000 –max-length 1000000 –min-rq 0.99”. .. Additionally, Hi-C libraries were constructed using fresh leaf tissue fixed in 1% formaldehyde to extract chromatin, which was digested using the DpnII restriction enzyme (New England Biolabs, USA). .. After quality control, the Hi-C libraries were sequenced on an Illumina NovaSeq X platform (Illumina, CA, USA), yielding a total of 72.17 Gb Hi-C data.

    Article Title: High‐Resolution Genomic Resources for Trait Mapping and Precision Breeding for Adzuki Bean ( Vigna angularis )
    Article Snippet: For sequencing the genome of “ZH20”, a 15 Kb library was constructed and sequenced using the PacBio Sequel II platform (Pacific Biosciences, CA, USA), generating a total of 41.36 Gb CCS HiFi reads with an N50 size of 22.31 Kb using CCS software (v3.0.0) ( https://github.com/pacificbiosciences/unanimity/ ) using parameters “–min‐passes 3 –min‐length 10000 –max‐length 1000000 –min‐rq 0.99”. .. Additionally, Hi‐C libraries were constructed using fresh leaf tissue fixed in 1% formaldehyde to extract chromatin, which was digested using the DpnII restriction enzyme (New England Biolabs, USA). .. After quality control, the Hi‐C libraries were sequenced on an Illumina NovaSeq X platform (Illumina, CA, USA), yielding a total of 72.17 Gb Hi‐C data.

    other:

    Article Title: The insertion of an ATTTC repeat in an Alu element hyperactivates a neurodevelopmental enhancer in spinocerebellar ataxia type 37
    Article Snippet: DpnII restriction enzyme , New England Biolabs (NEB) , Cat #R0543M.

    Control:

    Article Title: A split luciferase biosensing platform for detection and imaging of chromatin loops in individual live cells
    Article Snippet: .. A 5 μl aliquot of undigested control was stored, and nuclei were incubated overnight with 250 U of DpnII restriction enzyme (New England Biolabs). ..

    Article Title: A split luciferase biosensing platform for detection and imaging of chromatin loops in individual live cells.
    Article Snippet: .. A 5 μl aliquot of undigested control was stored, and nuclei were incubated overnight with 250 U of DpnII restriction enzyme (New England Biolabs). ..

    Incubation:

    Article Title: A split luciferase biosensing platform for detection and imaging of chromatin loops in individual live cells
    Article Snippet: .. A 5 μl aliquot of undigested control was stored, and nuclei were incubated overnight with 250 U of DpnII restriction enzyme (New England Biolabs). ..

    Article Title: A split luciferase biosensing platform for detection and imaging of chromatin loops in individual live cells.
    Article Snippet: .. A 5 μl aliquot of undigested control was stored, and nuclei were incubated overnight with 250 U of DpnII restriction enzyme (New England Biolabs). ..

    Article Title: Lamins and lineage-relevant transcription factors coordinate gene expression in lineage development
    Article Snippet: .. The reaction was incubated at 37°C for one hour, followed by the addition of 2.5 μL of DpnII restriction enzyme (NEB, R0543S). ..



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